GTF2H2C Antibody (PCRP-GTF2H2C-2C9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07154
![Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]](https://resources.bio-techne.com/images/products/GTF2H2C-Antibody-PCRP-GTF2H2C-2C9-Immunohistochemistry-Paraffin-NBP3-07154-img0004.jpg "Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]")
![Protein Array: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]](https://resources.bio-techne.com/images/products/GTF2H2C-Antibody-PCRP-GTF2H2C-2C9-Protein-Array-NBP3-07154-img0001.jpg "Protein Array: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]")
![Immunocytochemistry/ Immunofluorescence: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]](https://resources.bio-techne.com/images/products/GTF2H2C-Antibody-PCRP-GTF2H2C-2C9-Immunocytochemistry-Immunofluorescence-NBP3-07154-img0003.jpg "Immunocytochemistry/ Immunofluorescence: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2C Clone # PCRP-GTF2H2C-2C9
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human GTF2H2C protein (Uniprot: Q6P1K8)
Reactivity Notes
Predicted to react in Mouse and Rat.
Localization
Nucleus. Nuclear speckles.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2C
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08896)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for GTF2H2C Antibody (PCRP-GTF2H2C-2C9)
Immunocytochemistry/ Immunofluorescence: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]
Immunocytochemistry/Immunofluorescence: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Immunofluorescence analysis of PFA-fixed HeLa cells. GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9) followed by goat anti- Mouse IgG-CF488 (green); phalloidin (red).![Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]](https://resources.bio-techne.com/images/products/GTF2H2C-Antibody-PCRP-GTF2H2C-2C9-Immunohistochemistry-Paraffin-NBP3-07154-img0005.jpg "Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]")
Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]
Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).![Flow Cytometry: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]](https://resources.bio-techne.com/images/products/GTF2H2C-Antibody-PCRP-GTF2H2C-2C9-Flow-Cytometry-NBP3-07154-img0002.jpg "Flow Cytometry: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]")
Flow Cytometry: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154]
Flow Cytometry: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).Applications for GTF2H2C Antibody (PCRP-GTF2H2C-2C9)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: GTF2H2C
Alternate Names
General transcription factor IIH polypeptide 2-like protein, general transcription factor IIH subunit 2-like protein, general transcription factor IIH, polypeptide 2C, GTF2H2C_2
Gene Symbol
GTF2H2C
UniProt
Additional GTF2H2C Products
Product Documents for GTF2H2C Antibody (PCRP-GTF2H2C-2C9)
Product Specific Notices for GTF2H2C Antibody (PCRP-GTF2H2C-2C9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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