GRP78/HSPA5 Antibody [DyLight 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06274G
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Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Sheep, Zebrafish
Cited:
Human
Predicted:
Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]
Reactivity Notes
Use in Zebrafish reported in scientific literature (PMID:34327238) C. elegans reactivity reported in scientific literature (PMID: 31398187).
Localization
Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Marker
ER Stress Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for GRP78/HSPA5 Antibody [DyLight 488]
Product Image: GRP78/HSPA5 Antibody [DyLight 488] [NBP1-06274G] - Vial of DyLight 488 conjugated antibody. DyLight 488 is optimally excited at 493 nm by the Blue laser (488 nm) and has an emission maximum of 518 nm.
Applications for GRP78/HSPA5 Antibody [DyLight 488]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Free-Floating
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP1-06274. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: GRP78/HSPA5
Long Name
75 KD Glucose Regulated Protein
Alternate Names
BIP, HSP70-5, HSPA5
Gene Symbol
HSPA5
Additional GRP78/HSPA5 Products
Product Documents for GRP78/HSPA5 Antibody [DyLight 488]
Product Specific Notices for GRP78/HSPA5 Antibody [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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