GRB2 Antibody (9K6H0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15638

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15638-100ul
NBP3-15638-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9K6H0 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRB2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 118-217 of human GRB2 (P62993). YFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GRB2 Antibody (9K6H0) (NBP3-15638) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GRB2 Antibody (9K6H0)

Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638]

Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638]

Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunoprecipitation: GRB2 Antibody (9K6H0) [NBP3-15638]

Immunoprecipitation: GRB2 Antibody (9K6H0) [NBP3-15638]

Immunoprecipitation: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of 300ug extracts of 293T cells using 3ug GRB2 antibody (NBP3-15638). Western blot was performed from the immunoprecipitate using GRB2 (NBP3-15638) at a dilition of 1:1000.
GRB2 Antibody (9K6H0)

Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (9K6H0) [GRB2] -

Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (9K6H0) [GRB2] - Confocal imaging of HeLa cells using GRB2 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
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Applications for GRB2 Antibody (9K6H0)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:100 - 1:800

Immunohistochemistry

1:200 - 1:800

Immunohistochemistry-Paraffin

1:200 - 1:800

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRB2

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Long Name

Growth Factor Receptor-Bound Protein 2

Alternate Names

ASH, EGFRBP-GRB2, Grb3-3

Gene Symbol

GRB2

Additional GRB2 Products

Product Documents for GRB2 Antibody (9K6H0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRB2 Antibody (9K6H0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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