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Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44182

Novus Biologicals, part of Bio-Techne
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NBP2-44182
NBP2-44182-0.05mg

Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoblotting, ELISA, Immunoprecipitation

Cited:

Immunoblotting

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 4D12.H7.D10.E3

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This Protein A purified Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) was prepared by repeated immunizations in mice with Glutathione S-Transferase/GST Protein

Reactivity Notes

Cross reactivity from other sources has not been determined

Specificity

Reacts specifically with Glutathione S-Transferase/GST protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free

Western Blot: Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) [NBP2-44182]

Western Blot: Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) [NBP2-44182]

Western Blot: Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) [NBP2-44182] - Western Blot of Mouse anti-GSTS1 Monoclonal Antibody. Lane 1: rGSTS1 protein. Lane 2: GST. Load: 50 ng per lane. Primary antibody: Mouse anti-GSTS1 Monoclonal Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated Rb-a-Ms IgG at 1:40,000 for 30 min at RT. Block: 0.75% Casein-TBS for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTS1.

Applications for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free

Application
Recommended Usage

ELISA

1:50000-1:200000

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:1000
Application Notes
This product has been tested by ELISA, SDS-Page, and Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Use in immunoblotting reported in scientific literature (PMID: 27573245).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Glutathione S-Transferase/GST

Alternate Names

Glutathione STransferase

Additional Glutathione S-Transferase/GST Products

Product Documents for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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