Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44182
Key Product Details
Species Reactivity
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot, Immunoblotting, ELISA, Immunoprecipitation
Cited:
Immunoblotting
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # 4D12.H7.D10.E3
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Protein A purified Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) was prepared by repeated immunizations in mice with Glutathione S-Transferase/GST Protein
Reactivity Notes
Cross reactivity from other sources has not been determined
Specificity
Reacts specifically with Glutathione S-Transferase/GST protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Description
This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free
Western Blot: Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) [NBP2-44182]
Western Blot: Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) [NBP2-44182] - Western Blot of Mouse anti-GSTS1 Monoclonal Antibody. Lane 1: rGSTS1 protein. Lane 2: GST. Load: 50 ng per lane. Primary antibody: Mouse anti-GSTS1 Monoclonal Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated Rb-a-Ms IgG at 1:40,000 for 30 min at RT. Block: 0.75% Casein-TBS for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTS1.Applications for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free
Application
Recommended Usage
ELISA
1:50000-1:200000
Immunohistochemistry
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500-1:1000
Application Notes
This product has been tested by ELISA, SDS-Page, and Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.
Use in immunoblotting reported in scientific literature (PMID: 27573245).
Use in immunoblotting reported in scientific literature (PMID: 27573245).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Glutathione S-Transferase/GST
Alternate Names
Glutathione STransferase
Additional Glutathione S-Transferase/GST Products
Product Documents for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free
Product Specific Notices for Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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