GLIS3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33787
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SPGHNVQGSPHNPSSQLPPLTAVDAGAERFAPSAPSPHHISPRRVPAPSSILQRTQPPYTQQPSGSHLKSYQPETNSSFQPNGI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit GLIS3 Antibody - BSA Free (NBP2-33787) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-GLIS3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GLIS3 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: GLIS3 Antibody [NBP2-33787]](https://resources.bio-techne.com/images/products/GLIS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33787-img0008.jpg "Immunocytochemistry/ Immunofluorescence: GLIS3 Antibody [NBP2-33787]")
Immunocytochemistry/ Immunofluorescence: GLIS3 Antibody [NBP2-33787]
Immunocytochemistry/Immunofluorescence: GLIS3 Antibody [NBP2-33787] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: GLIS3 Antibody [NBP2-33787]](https://resources.bio-techne.com/images/products/GLIS3-Antibody-Immunohistochemistry-Paraffin-NBP2-33787-img0007.jpg "Immunohistochemistry-Paraffin: GLIS3 Antibody [NBP2-33787]")
Immunohistochemistry-Paraffin: GLIS3 Antibody [NBP2-33787]
Immunohistochemistry-Paraffin: GLIS3 Antibody [NBP2-33787] - Staining of human thyroid gland shows moderate nuclear positivity in glandular cells.![GLIS3 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: GLIS3 Antibody - BSA Free [NBP2-33787]](https://resources.bio-techne.com/images/products/nbp2-33787_rabbit-polyclonal-glis3-antibody-256202591390.jpg "Chromatin Immunoprecipitation-exo-Seq: GLIS3 Antibody - BSA Free [NBP2-33787]")
Chromatin Immunoprecipitation-exo-Seq: GLIS3 Antibody - BSA Free [NBP2-33787]
ChIP-Exo-Seq composite graph for Anti-GLIS3 (NBP2-33787) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for GLIS3 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLIS3
Alternate Names
FLJ38999, FLJ90578, GLIS family zinc finger 3, GLI-similar 3, OK/KNS-cl.4, zinc finger protein 515, zinc finger protein GLIS3
Gene Symbol
GLIS3
UniProt
Additional GLIS3 Products
Product Documents for GLIS3 Antibody - BSA Free
Product Specific Notices for GLIS3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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