GLI-2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23602
![Immunohistochemistry: GLI-2 Antibody - BSA Free [NBP2-23602]](https://resources.bio-techne.com/images/products/GLI-2-Antibody-Immunohistochemistry-NBP2-23602-img0005.jpg "Immunohistochemistry: GLI-2 Antibody - BSA Free [NBP2-23602]")
![Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]](https://resources.bio-techne.com/images/products/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0007.jpg "Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Predicted:
Hamster (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Frozen, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human GLI-2 protein (between residues 300-400) [UniProt# P10070]
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 26210874).
Localization
Nuclear and cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GLI-2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]
Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.![Immunohistochemistry-Paraffin: GLI-2 Antibody - BSA Free [NBP2-23602]](https://resources.bio-techne.com/images/products/GLI-2-Antibody-Immunohistochemistry-Paraffin-NBP2-23602-img0006.jpg "Immunohistochemistry-Paraffin: GLI-2 Antibody - BSA Free [NBP2-23602]")
Immunohistochemistry-Paraffin: GLI-2 Antibody - BSA Free [NBP2-23602]
Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.![Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]](https://resources.bio-techne.com/images/products/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0004.jpg "Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]")
Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]
Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.Applications for GLI-2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:200 - 1:300
Immunohistochemistry-Frozen
reported in scientific literature (PMID 26210874)
Immunohistochemistry-Paraffin
1:200 - 1:300
Application Notes
In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 review rated 4 using NBP2-23602 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLI-2
Long Name
GLI-Kruppel family member GLI2
Alternate Names
GLI2, THP2
Gene Symbol
GLI2
UniProt
Additional GLI-2 Products
Product Documents for GLI-2 Antibody - BSA Free
Product Specific Notices for GLI-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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