Girdin [p Ser1674] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23205
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-Girdin (Ser-1674) synthetic peptide (coupled to carrier) corresponding to amino acid residues around serine 1674 in human Girdin. This sequence has high homology to rat and mouse Girdin, and has low homology to similar regions in other Girdin family members. [UniProt# Q3V6T2]
Modification
p Ser1674
Specificity
The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1674) peptide before affinity purification using phospho-Girdin (Ser-1674) peptide. This antibody detects a 250 kDa protein on SDS-PAGE immunoblots of human A431 cells stimulated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylate Girdin.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Girdin [p Ser1674] Antibody
![Girdin [p Ser1674] Antibody](https://resources.bio-techne.com/images/products/nbp3-23205_rabbit-girdin-p-ser1674-pab-199202317594420.jpg "Western Blot: Girdin [p Ser1674] Antibody [NBP3-23205] -")
Western Blot: Girdin [p Ser1674] Antibody [NBP3-23205] -
Western Blot: Girdin [p Ser1674] Antibody [NBP3-23205] - Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1674), phospho-specific (lanes 3 & 4).Applications for Girdin [p Ser1674] Antibody
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Girdin
Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
Long Name
Girders of Actin Filament
Alternate Names
APE, CCDC88A, GIV, GRDN, HkRP1
Gene Symbol
CCDC88A
Additional Girdin Products
Product Documents for Girdin [p Ser1674] Antibody
Product Specific Notices for Girdin [p Ser1674] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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