Gelsolin/GSN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53814

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NB100-53814
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Gelsolin/GSN Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PRLKDKKMDAHP corresponding to internal region according to NP_937895.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Gelsolin/GSN Antibody

Western Blot: Gelsolin/GSN Antibody [NB100-53814]

Western Blot: Gelsolin/GSN Antibody [NB100-53814]

Western Blot: Gelsolin/GSN Antibody [NB100-53814] - Staining of Human Colorectal cancer lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: Gelsolin/GSN Antibody [NB100-53814]

Flow Cytometry: Gelsolin/GSN Antibody [NB100-53814]

Flow Cytometry: Gelsolin/GSN Antibody [NB100-53814] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Gelsolin/GSN Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium dependent actin binding protein. It is a potent modulator of actin filament length and gelation. Gelsolin has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called actin depolymerizing factor (ADF) or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25 amino acid residue extension at the NH2 terminus in the human plasma gelsolin which appears to account for the difference in relative molecular weights.

Alternate Names

ADF, AGEL, Brevin, GSN

Entrez Gene IDs

2934 (Human); 227753 (Mouse); 296654 (Rat)

Gene Symbol

GSN

UniProt

NP_000168.1 (Human), NP_937895.1 (Human)

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Gelsolin/GSN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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