GCNT3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88313
![Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunohistochemistry-Paraffin-NBP1-88313-img0017.jpg "Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]")
![Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunohistochemistry-Paraffin-NBP1-88313-img0015.jpg "Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFER
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 24889196).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GCNT3 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88313-img0014.jpg "Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]")
Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]
Immunocytochemistry/Immunofluorescence: GCNT3 Antibody [NBP1-88313] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.![Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunohistochemistry-Paraffin-NBP1-88313-img0016.jpg "Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]")
Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]
Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining of human rectum shows high expression.![GCNT3 Antibody - BSA Free Western Blot: GCNT3 Antibody - BSA Free [NBP1-88313]](https://resources.bio-techne.com/images/products/nbp1-88313_rabbit-polyclonal-gcnt3-antibody-65202516193624.jpg "Western Blot: GCNT3 Antibody - BSA Free [NBP1-88313]")
![Simple Western: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Simple-Western-NBP1-88313-img0007.jpg "Simple Western: GCNT3 Antibody [NBP1-88313]")
![Simple Western: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Simple-Western-NBP1-88313-img0008.jpg "Simple Western: GCNT3 Antibody [NBP1-88313]")
![Simple Western: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Simple-Western-NBP1-88313-img0009.jpg "Simple Western: GCNT3 Antibody [NBP1-88313]")
Applications for GCNT3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24889196). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 61-64 kDa
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 61-64 kDa
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GCNT3
Alternate Names
beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3, beta1,6GlcNAc-transferase, beta1,6-N-acetylglucosaminyltransferase-M, C2/4GnTGNTM, C24GNT, C2GnT2, C2GNTM, C2GnT-M, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase, EC 2.4.1.102, EC 2.4.1.150, glucosaminyl (N-acetyl) transferase 3, mucin type, hC2GnT-M, mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
Gene Symbol
GCNT3
Additional GCNT3 Products
Product Documents for GCNT3 Antibody - BSA Free
Product Specific Notices for GCNT3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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