GABA-A R beta 3 Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-199

Novus Biologicals, part of Bio-Techne
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NB300-199
NB300-199-0.02ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA-A R beta 3 Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytoplasmic loop of the beta 3 subunit. Accession # P63079

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 21901840).

Specificity

Specific for the ~53 kDa beta 3-subunit of the GABAA receptor in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GABA-A R beta 3 Antibody - Azide Free (NB300-199) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-GABA-A R beta 3 Antibody: Cited in 19 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GABA-A R beta 3 Antibody - Azide Free

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Immunostaining of mouse retina showing specific labeling of the GABAA beta3 subunit in green, calbindin in red and DNA in blue.
Western Blot: GABA-A R beta 3 Antibody [NB300-199]

Western Blot: GABA-A R beta 3 Antibody [NB300-199]

GABA-A-R-beta-3-Antibody-Western-Blot-NB300-199-img0010.jpg
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

GABA-A-R-beta-3-Antibody-Immunohistochemistry-NB300-199-img0011.jpg
View 3 more images

Applications for GABA-A R beta 3 Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:300

Simple Western

1:100

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry free-floating reported in scientific literature (PMID: 26592770). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17021187). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23430456). See Simple Western Antibody Database for Simple Western validation: Human Brain lysate at 0.5 mg/ml as sample; separated by size; antibody dilution of 1:100; observed molecular weight was 48 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-199 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/ml BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R beta 3

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide

Alternate Names

GABAAR beta 3, GABAARb3, GABRB3

Entrez Gene IDs

2562 (Human); 24922 (Rat)

Gene Symbol

GABRB3

UniProt

P63079 (Rat)

Additional GABA-A R beta 3 Products

Product Documents for GABA-A R beta 3 Antibody - Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-A R beta 3 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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