G-CSFR/CD114 Antibody (PSH03-44)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32378
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-44 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human G-CSFR/CD114 aa 651-836 / 836. (Uniprot: Q99062)
Localization
Cell membrane; Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit G-CSFR/CD114 Antibody (PSH03-44) (NBP3-32378) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for G-CSFR/CD114 Antibody (PSH03-44)
Western Blot: G-CSFR/CD114 Antibody (PSH03-44) [NBP3-32378]
Western Blot: G-CSFR/CD114 Antibody (PSH03-44) [NBP3-32378] - Western blot analysis of G-CSFR/CD114 on different lysates with Rabbit anti-G-CSFR/CD114 antibody (NBP3-32378) at 1/2,000 dilution.Lane 1: A431 cell lysate (20 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: THP-1 cell lysate (no heat) (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: Ramos cell lysate (20 ug/Lane)
Lane 6: Mouse placenta tissue lysate (40 ug/Lane)
Lane 7: Mouse brain tissue lysate (no heat) (40 ug/Lane)
Lane 8: Mouse testis tissue lysate (40 ug/Lane)
Lane 9: Rat placenta tissue lysate (40 ug/Lane)
Lane 10: Rat brain tissue lysate (no heat) (40 ug/Lane)
Lane 11: Rat testis tissue lysate (40 ug/Lane)
Lane 12: Rat skin tissue lysate (no heat) (40 ug/Lane)
Predicted band size: 92 kDa
Observed band size: 50-110 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for G-CSFR/CD114 Antibody (PSH03-44)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: G-CSFR/CD114
Long Name
Granulocyte Colony Stimulating Factor Receptor
Alternate Names
CD114, CSF3R, Csfgr, G-CSF R, GCSFR
Gene Symbol
CSF3R
Additional G-CSFR/CD114 Products
Product Documents for G-CSFR/CD114 Antibody (PSH03-44)
Product Specific Notices for G-CSFR/CD114 Antibody (PSH03-44)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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