FoxP3 Antibody (3G3) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33297PE

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NBP2-33297PE
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FoxP3 Antibodies »

Product Specifications

Immunogen

Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for FoxP3 Antibody (3G3) [PE]

Flow Cytometry: FoxP3 Antibody (3G3) [PE] [NBP2-33297PE] - Analysis in 2x10^6 mouse lymph node cells using 0.1 ug of antibody.
Flow Cytometry: FoxP3 Antibody (3G3) [PE] [NBP2-33297PE]

Flow Cytometry: FoxP3 Antibody (3G3) [PE] [NBP2-33297PE]

Flow Cytometry: FOXP3 Antibody (3G3) [NBP2-33297PE] - Analysis in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti CD25 APC conjugate, anti FOXP3 PE conjugate (NBP2-33297PE).

Applications for FoxP3 Antibody (3G3) [PE]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-33297. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FoxP3

One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FoxP3, a transcription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FoxP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FoxP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID, anti-human foxp3 pe

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for FoxP3 Antibody (3G3) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FoxP3 Antibody (3G3) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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