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FoxM1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74660

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 713 and 763 of human forkhead box M1 using the numbering given in entry NP_973731.1 (GeneID 2305).

Localization

Nuclear

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FoxM1 Antibody - BSA Free (NB100-74660) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FoxM1 Antibody - BSA Free

Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660] - Detection of Human FOXM1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FOXM1 antibody NB100-74660 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660]

Western Blot: FoxM1 Antibody [NB100-74660] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74660 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. FoxM1 was successfully immunoprecipitated by another rabbit anti-FoxM1 antibodies and NB100-74660, which recognize upstream epitopes.

Applications for FoxM1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FoxM1

FOXM1 belongs to a large family of Forkhead transcription factors that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). It is a transcription factor critical to cell cycle progression via its role in coordinating the G2/M phase transition. FOXM1 is ubiquitously expressed in proliferating cells but is absent in differentiated cells. FOXM1 overexpression has been detected in a variety of tumor types including breast, lung, prostate, and liver cancer, and appears to be involved in tumorigenesis-associated processes such as angiogenesis and metastasis. In light of these findings, FOXM1 has recently become an attractive target for anti-cancer therapy.

Long Name

Forkhead Box M1

Alternate Names

FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP-2, PIG29, Trident

Entrez Gene IDs

2305 (Human)

Gene Symbol

FOXM1

UniProt

Additional FoxM1 Products

Product Documents for FoxM1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FoxM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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