Flagellin Antibody (06) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05967
![Western Blot: Flagellin Antibody (06) [NBP3-05967]](https://resources.bio-techne.com/images/products/Flagellin-Antibody-06-Western-Blot-NBP3-05967-img0002.jpg "Western Blot: Flagellin Antibody (06) [NBP3-05967]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Bacteria
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 06
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Listeria monocytogenes Flagellin (Accession#: YP_013330.1; Met1-Ser287).
Reactivity Notes
Reacts with Listeria monocytogenes
No cross-reactivity in ELISA with:
E. coli cell lysate
No cross-reactivity in ELISA with:
E. coli cell lysate
Specificity
No cross-reactivity in ELISA with: E. coli cell lysate
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Flagellin Antibody (06) - Azide and BSA Free
Western Blot: Flagellin Antibody (06) [NBP3-05967]
Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 10ng. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.![Western Blot: Flagellin Antibody (06) [NBP3-05967]](https://resources.bio-techne.com/images/products/Flagellin-Antibody-06-Western-Blot-NBP3-05967-img0001.jpg "Western Blot: Flagellin Antibody (06) [NBP3-05967]")
Western Blot: Flagellin Antibody (06) [NBP3-05967]
Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 200ngLane B: 50ng. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Applications for Flagellin Antibody (06) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:1000-1:2000
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Flagellin
Additional Flagellin Products
Product Documents for Flagellin Antibody (06) - Azide and BSA Free
Product Specific Notices for Flagellin Antibody (06) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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