FKBP25 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87929
![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.bio-techne.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0007.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPKKDIIKFLQEHGSDSFLAEHKLLGNIKNVAKTANKDHLVTAYNHLFETKRFKGTESISKVSEQVKNVKLNEDKPKETKSEETLDEGPPKYTKSVLKKGDKTNFPKKGDVVHCWYTGTLQDGT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit FKBP25 Antibody - BSA Free (NBP1-87929) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FKBP25 Antibody - BSA Free
![FKBP25 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: FKBP25 Antibody - BSA Free [NBP1-87929]](https://resources.bio-techne.com/images/products/nbp1-87929_rabbit-polyclonal-fkbp25-antibody-65202516213814.jpg "Immunocytochemistry/ Immunofluorescence: FKBP25 Antibody - BSA Free [NBP1-87929]")
Immunocytochemistry/ Immunofluorescence: FKBP25 Antibody - BSA Free [NBP1-87929]
Staining of human cell line U-2 OS shows localization to cytosol.![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.bio-techne.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0008.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.bio-techne.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0006.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]
Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.![Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]](https://resources.bio-techne.com/images/products/FKBP25-Antibody-Immunohistochemistry-Paraffin-NBP1-87929-img0009.jpg "Immunohistochemistry-Paraffin: FKBP25 Antibody [NBP1-87929]")
![FKBP25 Antibody - BSA Free Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]](https://resources.bio-techne.com/images/products/nbp1-87929_rabbit-polyclonal-fkbp25-antibody-65202516262611.jpg "Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]")
![FKBP25 Antibody - BSA Free Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]](https://resources.bio-techne.com/images/products/nbp1-87929_rabbit-polyclonal-fkbp25-antibody-65202516264323.jpg "Western Blot: FKBP25 Antibody - BSA Free [NBP1-87929]")
Applications for FKBP25 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FKBP25
FK506 binding protein, 25 kDa molecular weight (FKBP25), also called FKBP3, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant Rapamycin is a potent inhibitor of FKBP25. FKBP25 is localized in the nucleus, where it alters the activity of histone deacetylases and transcription factors. FKBP25 is found in many tissues, including the brain, thymus, and spleen.
Long Name
25 kDa FK506 Binding Protein
Alternate Names
FKBP3
Gene Symbol
FKBP3
Additional FKBP25 Products
Product Documents for FKBP25 Antibody - BSA Free
Product Specific Notices for FKBP25 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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