FITC Antibody

Name: FITC Antibody
Brand: R&D Systems
SKU: G-148-C

R&D Systems, part of Bio-Techne | Catalog # G-148-C

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Product Datasheet / COA / SDS

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Mouse

Applications

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all FITC Antibodies »

Product Specifications

Immunogen

FITC-KLH (Fluorescein-isothiocyanate-coupled Keyhole Limpet Hemocyanin)

Specificity

Detects Fluorescein Isothiocyanate (FITC) in direct ELISAs. In direct ELISAs, nocross-reactivity with Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647,Alexa Fluor® 700, Alexa Fluor® 750, NorthernLights™ NL493, NorthernLights™ 557,and NorthernLights™ 637 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL

Formulation

Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl and Sodium Azide with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FITC

Fluorescein isothiocyante (FITC) is a derivative of fluorescein modified with an isothiocyanate reactive group (-N=C=S). FITC is often used to label antibodies for immunofluorescent cytochemistry and histochemistry. FITC can be also conjugated to proteins and RNA and DNA probes because such conjugation does not impair biological activity of these molecules. In some experimental setups FITC fluorescence may need to be enhanced, which is done by employing anti-FITC antibodies conjugated either to FITC or other fluorophores with similar excitation and emission spectra.

Long Name

Fluorescein Isothiocyanate

Alternate Names