Fibulin 2 Antibody - BSA Free

Immunohistochemistry: Fibulin 2 Antibody - BSA Free [NBP1-33479] -

ITGBL1 negatively regulated FBLN2 expression (A). Heatmap of DEGs in AR‐AGS and ITGBL1‐overexpressing AR‐AGS cells after 48 h of suspension culture. (B) Volcano plot of DEGs in AR‐AGS and ITGBL1‐overexpressing AR‐AGS cells after 48 h of suspension culture. (C) Overlap analysis of DEGs between the LASSO technique and SVM algorithm. (D) The protein expression of FBLN2 and ITGBL1 in WT and AR‐GC cells. GAPDH served as a loading control. The quantification of Western blot band densities was performed using the ImageJ program. (E) The protein expression of FBLN2 in ITGBL1‐overexpressing AR‐AGS cells and ITGBL1‐knockdown AR‐MKN45 cells after 48 h of suspension culture. GAPDH served as a loading control. The quantification of Western blot band densities was performed using the ImageJ program. (F) Typical images of stage M0 and M1 GC patients' tissues stained with FBLN2 by IHC. Scale bar, 50 μm. (G) Typical images of ITGBL1 and FBLN2 IHC staining in tissue from GC patient #5 are shown. Scale bar, 50 μm. (H) Correlation analysis between ITGBL1 and FBLN2 expression in GC tissues (R = −0.2103, p = 0.0466). (I) Kaplan–Meier curves of overall survival of GC patients classified into four subgroups based on ITGBL1 and FBLN2 expression. The experiments were conducted in triplicate. The values were represented as means with standard deviations (SD), and the statistical significance was assessed using Student's t‐test. Nonsignificant results were denoted as ‘ns’, while significance levels were shown as *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38332530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Fibulin 2 Antibody - BSA Free [NBP1-33479] -

The ITGBL1/FBLN2 axis promoted lung metastasis of AR‐GC cells in vivo. (A) Images of mouse lungs after transplantation with AR‐MKN45 cells and ITGBL1‐knockdown AR‐MKN45 cells. (B) Typical images of haematoxylin–eosin staining in lung metastases from the sh‐NC and sh‐ITGBL1 groups. Scale bar, 50 μm. (C) The expression of ITGBL1, FBLN2, AKT, and P‐AKT in mouse lung tissues in the sh‐NC group and the sh‐ITGBL1 group was determined by IHC experiments. Scale bar, 50 μm. (D) The protein expression of FBLN2 in ITGBL1‐knockdown AR‐MKN45 cells after infection with lentivirus carrying FBLN2 siRNA. GAPDH served as a loading control. The quantification of Western blot band densities was performed using the ImageJ program. (E) Images of mouse lungs after transplantation with ITGBL1‐knockdown AR‐MKN45 cells and ITGBL1/FBLN2 double‐knockdown AR‐MKN45 cells. (F) Typical images of haematoxylin–eosin staining in lung metastases from the sh‐ITGBL1 and sh‐ITGBL1 + sh‐FBLN2 groups. Scale bar, 50 μm. (G) The expression of ITGBL1, FBLN2, AKT, and P‐AKT in mouse lung tissues in the sh‐ITGBL1 group and the sh‐ITGBL1 + sh‐FBLN2 group was determined by IHC experiments. Scale bar, 50 μm. (H) Diagrammatic representation of the ITGBL1/AKT/FBLN2 axis's regulation mechanisms in GC anoikis resistance and metastasis. The experiments were conducted in triplicate. The values were represented as means with standard deviations (SD), and the statistical significance was assessed using Student's t‐test. Nonsignificant results were denoted as ‘ns’, while significance levels were shown as *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38332530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Fibulin 2 Antibody - BSA Free [NBP1-33479] -

The ITGBL1/FBLN2 axis promoted lung metastasis of AR‐GC cells in vivo. (A) Images of mouse lungs after transplantation with AR‐MKN45 cells and ITGBL1‐knockdown AR‐MKN45 cells. (B) Typical images of haematoxylin–eosin staining in lung metastases from the sh‐NC and sh‐ITGBL1 groups. Scale bar, 50 μm. (C) The expression of ITGBL1, FBLN2, AKT, and P‐AKT in mouse lung tissues in the sh‐NC group and the sh‐ITGBL1 group was determined by IHC experiments. Scale bar, 50 μm. (D) The protein expression of FBLN2 in ITGBL1‐knockdown AR‐MKN45 cells after infection with lentivirus carrying FBLN2 siRNA. GAPDH served as a loading control. The quantification of Western blot band densities was performed using the ImageJ program. (E) Images of mouse lungs after transplantation with ITGBL1‐knockdown AR‐MKN45 cells and ITGBL1/FBLN2 double‐knockdown AR‐MKN45 cells. (F) Typical images of haematoxylin–eosin staining in lung metastases from the sh‐ITGBL1 and sh‐ITGBL1 + sh‐FBLN2 groups. Scale bar, 50 μm. (G) The expression of ITGBL1, FBLN2, AKT, and P‐AKT in mouse lung tissues in the sh‐ITGBL1 group and the sh‐ITGBL1 + sh‐FBLN2 group was determined by IHC experiments. Scale bar, 50 μm. (H) Diagrammatic representation of the ITGBL1/AKT/FBLN2 axis's regulation mechanisms in GC anoikis resistance and metastasis. The experiments were conducted in triplicate. The values were represented as means with standard deviations (SD), and the statistical significance was assessed using Student's t‐test. Nonsignificant results were denoted as ‘ns’, while significance levels were shown as *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38332530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.