FHL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1461

Novus Biologicals, part of Bio-Techne
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NB100-1461
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all FHL1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CTVSRVSHPVSKARK corresponding to internal region according to AAC72886.1.

Specificity

This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat FHL1 Antibody (NB100-1461) is a polyclonal antibody validated for use in WB and ELISA. Anti-FHL1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FHL1 Antibody

Western Blot: FHL1 Antibody [NB100-1461]

Western Blot: FHL1 Antibody [NB100-1461]

Western Blot: FHL1 Antibody [NB100-1461] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: FHL1 Antibody [NB100-1461]

Western Blot: FHL1 Antibody [NB100-1461]

FHL1-Antibody-Western-Blot-NB100-1461-img0002.jpg

Applications for FHL1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

1:100-1:2000
Application Notes
WB: Approx. 30 kDa band observed in human heart lysates (calculated MW of 36.3 kDa band according to AAC72886) is consistent with scientific literature (PMID: 1048092).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FHL1

LIM proteins, named for 'LIN11, ISL1 and MEC3' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.

Long Name

Four and a Half LIM Domains Protein 1

Alternate Names

HLH1, HPLH1, KYOT, SLIM1, SLIMMER, XMPMA

Entrez Gene IDs

2273 (Human)

Gene Symbol

FHL1

UniProt

AAC72886. (Human)

Additional FHL1 Products

Product Documents for FHL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FHL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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