FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70731
![Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70731-img0004.jpg "Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
![Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Western-Blot-NBP2-70731-img0002.jpg "Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5C5
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human FGFR2(NP_000132) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]
Western Blot: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2.![Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70731-img0005.jpg "Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunocytochemistry/Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2.![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0009.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of paraffin-embedded Human breast tissue using anti-FGFR2 mouse monoclonal antibody.![Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Western-Blot-NBP2-70731-img0001.jpg "Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]")
![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0006.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0007.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0008.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0010.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
![Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.bio-techne.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70731-img0003.jpg "Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Applications for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:200 - 1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FGFR2
FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Gene Symbol
FGFR2
Additional FGFR2 Products
Product Documents for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Product Specific Notices for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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