FGFR1 Antibody (M17D10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2080
![Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0008.jpg "Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]")
![Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0011.jpg "Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M17D10
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human ectodomain of FGFr1b expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is within the sequence pro179 to lys195 in Ig loop II.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 8575296). Not yet tested in other species.
Localization
Cell Membrane
Specificity
Reacts with both alpha and beta isoforms.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for FGFR1 Antibody (M17D10) - BSA Free
Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]
Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0012.jpg "Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]")
Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]
Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] conjugated to Alexa Fluor 488 (NB100-2080AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Western Blot: FGFR1 Antibody (M17D10)BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17D10-Western-Blot-NB100-2080-img0004.jpg "Western Blot: FGFR1 Antibody (M17D10)BSA Free [NB100-2080]")
Western Blot: FGFR1 Antibody (M17D10)BSA Free [NB100-2080]
Western Blot: FGF R1 Antibody (M17D10) [NB100-2080] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody.![Flow Cytometry: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0010.jpg "Flow Cytometry: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]")
![Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0006.jpg "Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]")
![Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0007.jpg "Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]")
![Flow Cytometry: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0009.jpg "Flow Cytometry: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]")
Applications for FGFR1 Antibody (M17D10) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:500 - 1:1000
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Immunohistochemistry
5 ug/mL
Immunohistochemistry-Paraffin
5 ug/mL
Immunoprecipitation
1:10 - 1:500
Western Blot
1 ug/mL
Application Notes
This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FGFR1
FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
Long Name
Fibroblast Growth Factor Receptor 1
Alternate Names
CD331, FGF R1, Flt-2
Entrez Gene IDs
2260 (Human)
Gene Symbol
FGFR1
UniProt
Additional FGFR1 Products
Product Documents for FGFR1 Antibody (M17D10) - BSA Free
Product Specific Notices for FGFR1 Antibody (M17D10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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