FEN-1 Antibody (S01-3I5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19863
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S01-3I5 expressed in HEK293
Concentration
0.3 mg/ml
Product Specifications
Immunogen
A synthetic peptide of human FEN-1 (Uniprot # P39748)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit FEN-1 Antibody (S01-3I5) (NBP3-19863) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FEN-1 Antibody (S01-3I5)
![Western Blot: FEN-1 Antibody (S01-3I5) [NBP3-19863]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-S01-3I5-Western-Blot-NBP3-19863-img0001.jpg "Western Blot: FEN-1 Antibody (S01-3I5) [NBP3-19863]")
Western Blot: FEN-1 Antibody (S01-3I5) [NBP3-19863]
Western Blot: FEN-1 Antibody (S01-3I5) [NBP3-19863] - Western blot detection of FEN-1 in Hela, MCF-7, U251, HL-60 using NBP3-19863 (1:1000 diluted)Applications for FEN-1 Antibody (S01-3I5)
Application
Recommended Usage
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FEN-1
The FEN-1 enzyme is active in base excision repair (BER) pathways. Once the DNA glycosylase removes the desired nucleotide stretch, a DNA polymerase synthesizes the complementary nucleotides for replacement. To complete the DNA repair, the FEN-1 enzyme removes the extra flap of nucleotides and DNA ligase finishes the repair.
Long Name
Flap Endonuclease 1
Alternate Names
FEN1
Gene Symbol
FEN1
Additional FEN-1 Products
Product Documents for FEN-1 Antibody (S01-3I5)
Product Specific Notices for FEN-1 Antibody (S01-3I5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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