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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Feline

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PF6J-2A1

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Made to Viral FELINE LEUKAEMIA VIRUS p15E

Specificity

NB100-65808 recognizes an epitope within the amino-terminal domain of both the 20kDa full-length (including intracellular sequence), and the 15kDa truncated forms, of feline leukaemia virus (FeLV) p15e transmembrane envelope protein. Persistent infection of cats with the oncoretrovirus FeLV can result in the non-neoplastic condition known as FeLV-associated enteritis (FAE). Studies have shown strong expression of the major FeLV proteins p15e and gp70 in intestinal crypt epithelial cells of cats with FAE, compared to FeLV-positive cats without intestinal alterations, thereby suggesting a pathogenic role for these envelope proteins/precursors in FAE.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Feline Leukaemia Virus p15E Antibody (PF6J-2A1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:2000
Application Notes
In WB detects band/s of approximately 15kDa and 20kDa under reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

<0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Feline Leukaemia Virus p15E

FeLV is a retrovirus (family Oncovirinae) that can cause severe immunosuppression, severe non regenerative anemia, and a variety of neoplasms. The 3 main subtypes (FeLVA, FeLVB and FeLVC) may act independently or together to cause a variety of diseases. The major envelope glycoprotein (gp70) is used to bind to target cells and varies with the FeLV subtype.

Alternate Names

Env polyprotein, Feline Leukemia Virus glycoprotein 70, Glycoprotein gp70 [Precursor], GP70 envelope protein, gp70 protein (SU), p15E protein, p15E protein (TM)

UniProt

Additional Feline Leukaemia Virus p15E Products

Product Documents for Feline Leukaemia Virus p15E Antibody (PF6J-2A1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Feline Leukaemia Virus p15E Antibody (PF6J-2A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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