FAK [p Tyr397] Antibody (24GB5215)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50835
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB5215
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Phospho-FAK (Y397) (Uniprot: Q05397)
Modification
p Tyr397
Specificity
This antibody detects endogenous levels of PTK2 only when phosphorylated at Y397
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAK [p Tyr397] Antibody (24GB5215)
![FAK [p Tyr397] Antibody (24GB5215)](https://resources.bio-techne.com/images/products/nbp3-50835_-flow-cytometry-639228449246488782.png "Flow Cytometry: FAK [p Tyr397] Antibody (24GB5215)[NBP3-50835] -")
Flow Cytometry: FAK [p Tyr397] Antibody (24GB5215)[NBP3-50835] -
Flow cytometric analysis of FAKexpression in HepG2 cells using FAK antibody (1:2,000). Green, isotype control; red, phospho-FAK (Y397).![FAK [p Tyr397] Antibody (24GB5215)](https://resources.bio-techne.com/images/products/nbp3-50835_-western-blot-639228453506693006.png "Western Blot: FAK [p Tyr397] Antibody (24GB5215)[NBP3-50835] -")
Western Blot: FAK [p Tyr397] Antibody (24GB5215)[NBP3-50835] -
Western blotting analysis using FAK (Y397) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for FAK [p Tyr397] Antibody (24GB5215)
Application
Recommended Usage
Flow Cytometry
1:200-1:2,000
Immunocytochemistry/ Immunofluorescence
1:100-1:1,000
Western Blot
1:1,000-1:5,000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FAK
FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
Additional FAK Products
Product Documents for FAK [p Tyr397] Antibody (24GB5215)
Product Specific Notices for FAK [p Tyr397] Antibody (24GB5215)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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