espin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90588

Novus Biologicals, part of Bio-Techne
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NBP1-90588
NBP1-90588-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all espin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SMPAWRRDLLRKKLEEEREQKRKEEERQKQEELRREKEQSEKLRTLGYDESKLAPWQRQVILKK

Reactivity Notes

Rat reactivity reported in (PMID:30467855). Mouse (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit espin Antibody - BSA Free (NBP1-90588) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-espin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for espin Antibody - BSA Free

espin Antibody - BSA Free Immunohistochemistry: espin Antibody - BSA Free [NBP1-90588]

Immunohistochemistry: espin Antibody - BSA Free [NBP1-90588]

Analysis in human testis and skeletal muscle tissues using NBP1-90588 antibody. Corresponding ESPN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
View 5 more images

Applications for espin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:20

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:20, apparent MW was 146 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: espin

Alternate Names

autosomal recessive deafness type 36 protein, DKFZp434A196, DKFZp434G2126, espin

Entrez Gene IDs

83715 (Human)

Gene Symbol

ESPN

UniProt

B1AK53 (Human)

Additional espin Products

Product Documents for espin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for espin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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