Skip to main content

ErbB3/Her3 Antibody (PD00-44)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32313

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-32313

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-44 expressed in HEK293

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human ErbB3 aa 650-750 (internal sequence). (Uniprot: P21860)

Localization

Cell membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

148 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ErbB3/Her3 Antibody (PD00-44) (NBP3-32313) is a recombinant monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ErbB3/Her3 Antibody (PD00-44)

ErbB3/Her3 Antibody (PD00-44)

Immunohistochemistry: ErbB3/Her3 Antibody (PD00-44) [NBP3-32313]

Immunohistochemistry: ErbB3/Her3 Antibody (PD00-44) [NBP3-32313] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ErbB3/Her3 antibody (NBP3-32313) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32313) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for ErbB3/Her3 Antibody (PD00-44)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB3/Her3

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Gene Symbol

ERBB3

Additional ErbB3/Her3 Products

Product Documents for ErbB3/Her3 Antibody (PD00-44)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ErbB3/Her3 Antibody (PD00-44)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...