EPLIN Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87947

Novus Biologicals, part of Bio-Techne
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NBP1-87947
NBP1-87947-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EPLIN Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDS

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 24135282).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit EPLIN Antibody - BSA Free (NBP1-87947) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-EPLIN Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EPLIN Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP1-87947]

Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP1-87947]

Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NBP1-87947] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.
Western Blot: EPLIN Antibody [NBP1-87947]

Western Blot: EPLIN Antibody [NBP1-87947]

Western Blot: EPLIN Antibody [NBP1-87947] - Analysis using Anti-LIMA1 antibody NBP1-87947 (A) shows similar pattern to independent antibody NBP2-38673 (B).
EPLIN Antibody - BSA Free Western Blot: EPLIN Antibody - BSA Free [NBP1-87947]

Western Blot: EPLIN Antibody - BSA Free [NBP1-87947]

Analysis in human cell lines A-431 and A-549 using Anti-LIMA1 antibody. Corresponding LIMA1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
View 6 more images

Applications for EPLIN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EPLIN

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

Alternate Names

1110021C24Rik, Epithelial protein lost in neoplasm, EPLINepithelial protein lost in neoplasm beta, FLJ38853, LIM domain and actin binding 1, MGC131726, SREBP3LIM domain and actin-binding protein 1, sterol regulatory element binding protein 3

Entrez Gene IDs

51474 (Human)

Gene Symbol

LIMA1

UniProt

Q9UHB6 (Human)

Additional EPLIN Products

Product Documents for EPLIN Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EPLIN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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