Ephrin-B2 Antibody [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # AF1088A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Zebrafish
Applications
Western Blot
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant zebrafish Ephrin-B2
Leu25-Ala222
Accession # O73874
Leu25-Ala222
Accession # O73874
Specificity
Detects zebrafish Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Ephrin-B2 is observed and less than 1% cross-reactivity with recombinant human (rh) Ephrin-A4, rmEphrin-A2, rmEphrin-A1, rhEphrin-B3, rmEphrin-B1, rhEphrin-A3, rhEphrin-A5, and rmEphrin-A4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Ephrin-B2 Antibody [Allophycocyanin]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Ephrin-B2
Ephrins are naturally divided into two structural groups. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins, which can be tyrosine phosphorylated upon receptor ligation. Class B ephrins show 33% amino acid sequence identity in their extracellular segments and 44% amino acid sequence identity in their cytoplasmic regions.
Alternate Names
EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1
Gene Symbol
EFNB2
Additional Ephrin-B2 Products
Product Documents for Ephrin-B2 Antibody [Allophycocyanin]
Product Specific Notices for Ephrin-B2 Antibody [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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