Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Label

Janelia Fluor 549

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EphB1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat EphB1
Met18-Gln538
Accession # P09759

Specificity

Detects rat EphB1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA4, rmEphB2, rmEphB3, rmEphB4, and rmEphB6 is observed, less than 1% cross-reactivity with recombinant human (rh) EphA1, rhEphA5, rmEphA7, rmEphA8, and recombinant rat EphA5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for EphB1 Antibody [Janelia Fluor® 549]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EphB1

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

Long Name

Eph Receptor B1

Alternate Names

Cek6, Elk, Hek6, Net

Gene Symbol

EPHB1

Additional EphB1 Products

Product Documents for EphB1 Antibody [Janelia Fluor® 549]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EphB1 Antibody [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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