Eosinophil Antibody (BMK-13)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42140
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Canine, Monkey, Rhesus Macaque
Cited:
Mouse, Canine, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Flow Cytometry
Cited:
Immunohistochemistry-Paraffin, ELISA, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # BMK-13
Concentration
0.1 mg/ml
Product Specifications
Immunogen
The antibody reacts with the Eosinophil Major Basic Protein antigen.
Reactivity Notes
Use in Rhesus Macaque reported in scientific literature (PMID:32128254). Canine reactivity reported in scientific literature (PMID: 29920692). Monkey reactivity reported in scientific literature (PMID: 30626745). Mouse reactivity reported in scientific literature (PMID: 31643033, 31626785).
Specificity
Cross-reacts weakly with human basophils, which also contain low level of this protein. It does not cross-react with any other human protein or cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Eosinophil Antibody (BMK-13)
Flow Cytometry: Mouse Monoclonal Eosinophil Antibody (BMK-13) [NBP1-42140]
Flow Cytometry: Mouse Monoclonal Eosinophil Antibody (BMK-13) [NBP1-42140] - LPS stimulated eosinophil. WBC was blocked (RBC lysed whole blood) with 5 % BSA for 15 min and then incubated with NBP1-42140 (1:100) for 2 h at 4 °C. The cells were washed and stained secondary antibody Alexa 430 and analyzed in FACS. Image from a verified customer review.Applications for Eosinophil Antibody (BMK-13)
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
Validated for Flow from a verified customer review.
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Application Notes
This mAb is a very useful tool for clinical and experimental allergy studies. BMK-13 is a pan-eosinophil marker in both frozen sections and paraffin embedded tissues. It stains in bronchial sections 98% of the periphere blood eosinophils. Further, the antibody is most useful for immunohistochemistry on frozen sections is the APAAP method. BMK-13 also binds to eosinophils in paraffin-embedded tissue. A 1:30 dilution in PBS is recommended. Incubation time: 1 hour at RT. Enzymatic pretreatment is necessary. This antibody is suitable for blood smears (cytospins) if fixed with methanol-acetone mixture (1:1, v/v). It is unlikely, without permeabilization, that the BMK-13 is suitable for the flow cytometry because BMK-13 stains intracellular, stored proteins.
Reviewed Applications
Read 1 review rated 4 using NBP1-42140 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Eosinophil
Alternate Names
BMPG, Bone marrow proteoglycan, EMBP, Eosinophil granule major basic protein, Eosinophil major basic protein, MBP, MGC14537, MPG, Natural killer cell activator, Pregnancy associated major basic protein, PRG 2, Proteoglycan 2, Proteoglycan 2 bone marrow, Proteoglycan 2 preproprotein, Proteoglycan2
Additional Eosinophil Products
Product Documents for Eosinophil Antibody (BMK-13)
Product Specific Notices for Eosinophil Antibody (BMK-13)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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