Enterovirus 71 VP1 Antibody (GT9512) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13544
Key Product Details
Species Reactivity
Virus
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT9512
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.
Reactivity Notes
Enterovirus 71
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Enterovirus 71 VP1 Antibody (GT9512) - Azide and BSA Free
![Western Blot: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544]](https://resources.bio-techne.com/images/products/Enterovirus-71-VP1-Antibody-GT9512-Western-Blot-NBP3-13544-img0001.jpg "Western Blot: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544]")
Western Blot: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544]
Western Blot: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] - Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) diluted at 1:500.
ELISA: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] -
ELISA: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] - Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus 71 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) (1 ug/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
Western Blot: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] -
Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for Enterovirus 71 VP1 Antibody (GT9512) - Azide and BSA Free
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Enterovirus 71 VP1
Alternate Names
Capsid protein VP1, EV71 VP1, P1D, Virion protein 1
Additional Enterovirus 71 VP1 Products
Product Documents for Enterovirus 71 VP1 Antibody (GT9512) - Azide and BSA Free
Product Specific Notices for Enterovirus 71 VP1 Antibody (GT9512) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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