ENO3 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00266
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human ENO3 (Accession#: AAH17249.1; Met1-Lys434)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ENO3 Antibody - Azide and BSA Free
Western Blot: ENO3 Antibody [NBP3-00266] -
Western Blot: ENO3 Antibody [NBP3-00266] - NBP3-00266 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:47 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: ENO3 Antibody [NBP3-00266] -
Immunoprecipitation: ENO3 Antibody [NBP3-00266] - ENO3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00266 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00266, at 1:200 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 47 kDaApplications for ENO3 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ENO3
Alternate Names
2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, beta-enolase, EC 4.2.1, EC 4.2.1.11, Enolase 3, enolase 3 (beta, muscle), enolase 3, (beta, muscle), GSD13, MSE, Muscle-specific enolase, Skeletal muscle enolase
Gene Symbol
ENO3
Additional ENO3 Products
Product Documents for ENO3 Antibody - Azide and BSA Free
Product Specific Notices for ENO3 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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