EMP2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86847
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]](https://resources.bio-techne.com/images/products/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0013.jpg "Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DNAWWVGDEFFADVWRICTNNTNCTVINDSFQEYST
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit EMP2 Antibody - BSA Free (NBP1-86847) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-EMP2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for EMP2 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: EMP2 Antibody [NBP1-86847]](https://resources.bio-techne.com/images/products/EMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86847-img0008.jpg "Immunocytochemistry/ Immunofluorescence: EMP2 Antibody [NBP1-86847]")
Immunocytochemistry/ Immunofluorescence: EMP2 Antibody [NBP1-86847]
Immunocytochemistry/Immunofluorescence: EMP2 Antibody [NBP1-86847] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]](https://resources.bio-techne.com/images/products/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0015.jpg "Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]")
Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]
Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in glomeruli.![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]](https://resources.bio-techne.com/images/products/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0012.jpg "Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]")
Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]
Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human skin shows moderate to strong positivity in plasma membrane in keratinocytes.![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]](https://resources.bio-techne.com/images/products/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0016.jpg "Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847]")
![Western Blot: EMP2 Antibody [NBP1-86847]](https://resources.bio-techne.com/images/products/EMP2-Antibody-Western-Blot-NBP1-86847-img0005.jpg "Western Blot: EMP2 Antibody [NBP1-86847]")
![EMP2 Antibody - BSA Free Western Blot: EMP2 Antibody - BSA Free [NBP1-86847]](https://resources.bio-techne.com/images/products/nbp1-86847_rabbit-polyclonal-emp2-antibody-30420251391729.jpg "Western Blot: EMP2 Antibody - BSA Free [NBP1-86847]")
![EMP2 Antibody - BSA Free Western Blot: EMP2 Antibody - BSA Free [NBP1-86847]](https://resources.bio-techne.com/images/products/nbp1-86847_rabbit-polyclonal-emp2-antibody-304202513144916.jpg "Western Blot: EMP2 Antibody - BSA Free [NBP1-86847]")
Applications for EMP2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 5 using NBP1-86847 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EMP2
Epithelial membrane protein-2 (EMP2), a tetraspan protein known to associate with and modify surface expression of certain integrin isoforms, was identified as a prognostic biomarker in human endometrial cancer. EMP2 is expressed in the majority of ovarian tumors and may be a feasible drug target in vivo. EMP2 in several cell types plays a role in growth control, invasion, metastasis, and protein trafficking. It can associate with integrin alpha v beta 3 and FAK, and promote integrin-mediated FAK-Src activation.
Long Name
Epithelial Membrane Protein 2
Alternate Names
XMP
Gene Symbol
EMP2
Additional EMP2 Products
Product Documents for EMP2 Antibody - BSA Free
Product Specific Notices for EMP2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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