eIF4B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33284
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: TTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit eIF4B Antibody - BSA Free (NBP2-33284) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for eIF4B Antibody - BSA Free
![Western Blot: eIF4B Antibody [NBP2-33284]](https://resources.bio-techne.com/images/products/eIF4B-Antibody-Western-Blot-NBP2-33284-img0005.jpg "Western Blot: eIF4B Antibody [NBP2-33284]")
Western Blot: eIF4B Antibody [NBP2-33284]
Western Blot: eIF4B Antibody [NBP2-33284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp![Immunocytochemistry/ Immunofluorescence: eIF4B Antibody [NBP2-33284]](https://resources.bio-techne.com/images/products/eIF4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33284-img0008.jpg "Immunocytochemistry/ Immunofluorescence: eIF4B Antibody [NBP2-33284]")
Immunocytochemistry/ Immunofluorescence: eIF4B Antibody [NBP2-33284]
Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-33284] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.![Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284]](https://resources.bio-techne.com/images/products/eIF4B-Antibody-Immunohistochemistry-Paraffin-NBP2-33284-img0007.jpg "Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284]")
Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284]
Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.Applications for eIF4B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF4B
Long Name
Eukaryotic Translation Initiation Factor 4B
Alternate Names
Eif4a2
Gene Symbol
EIF4B
UniProt
Additional eIF4B Products
Product Documents for eIF4B Antibody - BSA Free
Product Specific Notices for eIF4B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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