EGFR [p Tyr1173] Antibody (EM-13) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44893
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Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Flow (Intracellular), Immunoprecipitation, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # EM-13
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.
Modification
p Tyr1173
Specificity
The antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine 1173.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for EGFR [p Tyr1173] Antibody (EM-13) - BSA Free
Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.
![EGFR [p Tyr1173] Antibody (EM-13) - BSA Free Flow Cytometry: EGFR [p Tyr1173] Antibody (EM-13) - BSA Free [NBP1-44893]](https://resources.bio-techne.com/images/products/nbp1-44893_mouse-monoclonal-egfr-p-tyr1173-antibody-em-13-13120241659357.jpg "Flow Cytometry: EGFR [p Tyr1173] Antibody (EM-13) - BSA Free [NBP1-44893]")
Flow Cytometry: EGFR [p Tyr1173] Antibody (EM-13) - BSA Free [NBP1-44893]
Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY1173) (EM-13) purified antibody (concentration in sample 3 ug/ml, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in Analysis (intracellular staining).Applications for EGFR [p Tyr1173] Antibody (EM-13) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
1-4 ug/ml
Immunoprecipitation
1:50
Western Blot
1 ug/ml
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EGFR
In addition to its role in normal development, EGFR mutations or overexpression is observed in many tumors, including breast cancer, non-small cell lung carcinoma (NSCLC), colon cancer, and more (3-6). Small molecule tyrosine kinase inhibitors (TKIs), like gefitinib, erlotinib, and afatinib, have shown great efficacy in treating patients with EGFR activating mutations, especially for NSCLC (4-6). However, most patients eventually develop acquired resistance to TKIs and thus combination and alternative therapies are in development (4-6). A third-generation TKI, osimertinib, is approved for NSCLC patients with resistance to first-line EGFR TKI treatment (6). Additionally, combination therapies of EGFR TKIs with monoclonal antibody immunotherapies, like anti-PD-L1, are being further investigated in clinical trials (6).
References
1. Roskoski R Jr. Small molecule inhibitors targeting the EGFR/ErbB family of protein-tyrosine kinases in human cancers. Pharmacol Res. 2019; 139:395-411. https://doi.org/10.1016/j.phrs.2018.11.014
2. Sigismund S, Avanzato D, Lanzetti L. Emerging functions of the EGFR in cancer. Mol Oncol. 2018; 12(1):3-20. https://doi.org/10.1002/1878-0261.12155
3. Normanno N, De Luca A, Bianco C, et al. Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 2006; 366(1):2-16. https://doi.org/10.1016/j.gene.2005.10.018
4. Liu Q, Yu S, Zhao W, Qin S, Chu Q, Wu K. EGFR-TKIs resistance via EGFR-independent signaling pathways. Mol Cancer. 2018; 17(1):53. https://doi.org/10.1186/s12943-018-0793-1
5. Harrison PT, Vyse S, Huang PH. Rare epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer. Semin Cancer Biol. 2020; 61:167-179. https://doi.org/10.1016/j.semcancer.2019.09.015
6. Wu SG, Shih JY. Management of acquired resistance to EGFR TKI-targeted therapy in advanced non-small cell lung cancer. Mol Cancer. 2018; 17(1):38. https://doi.org/10.1186/s12943-018-0777-1
Long Name
Epidermal Growth Factor Receptor
Alternate Names
EGF R, ErbB, ErbB1, HER-1
Gene Symbol
EGFR
Additional EGFR Products
Product Documents for EGFR [p Tyr1173] Antibody (EM-13) - BSA Free
Product Specific Notices for EGFR [p Tyr1173] Antibody (EM-13) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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