ECH1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00324
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human ECH1 (Uniprot#: Q13011; Thr34-Leu328)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ECH1 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] -
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human prostate with NBP3-00324 (1:10000, formalin-fixed paraffin embedded sections).
Western Blot: ECH1 Antibody [NBP3-00324] -
Western Blot: ECH1 Antibody [NBP3-00324] - NBP3-00324 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:34 kDa
Immunoprecipitation: ECH1 Antibody [NBP3-00324] -
Immunoprecipitation: ECH1 Antibody [NBP3-00324] - ECH1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00324 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00324, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size: 34 kDaApplications for ECH1 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry-Paraffin
1:2500-1:10000
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ECH1
Alternate Names
delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, delta3,5-delta2,4-dienoyl-CoA isomerase, dienoyl-CoA isomerase, EC 5.3.3.-, enoyl CoA hydratase 1, peroxisomal, enoyl Coenzyme A hydratase 1, peroxisomal, HPXEL, peroxisomal enoyl-CoA hydratase 1
Gene Symbol
ECH1
Additional ECH1 Products
Product Documents for ECH1 Antibody - Azide and BSA Free
Product Specific Notices for ECH1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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