EBOV GP Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12748
Key Product Details
Species Reactivity
Ebola Virus
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant EBOV GP (Accession#: AAC54887.1; Met1-Phe308)
Reactivity Notes
Ebola virus EBOV (subtype Zaire, strain Mayinga 1976)
Has cross-reactivity in WB with:
Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His Tag)(40442-V08B1)
Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) Glycoprotein / GP Protein
Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP Protein (aa:Met1-Asn637, His Tag)(40094-V08B1)
Has cross-reactivity in WB with:
Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His Tag)(40442-V08B1)
Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) Glycoprotein / GP Protein
Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP Protein (aa:Met1-Asn637, His Tag)(40094-V08B1)
Specificity
Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) GP-RBD / Glycoprotein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Scientific Data Images for EBOV GP Antibody - Azide and BSA Free
![Western Blot: EBOV GP Antibody [NBP3-12748]](https://resources.bio-techne.com/images/products/EBOV-GP-Antibody-Western-Blot-NBP3-12748-img0001.jpg "Western Blot: EBOV GP Antibody [NBP3-12748]")
Western Blot: EBOV GP Antibody [NBP3-12748]
Western Blot: EBOV GP Antibody [NBP3-12748] - Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) GP-RBD / Glycoprotein rabbit polyclonal antibody at 1:5000 dilutionLane A: Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP(10ng)Lane B: Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) GP-RBD / Glycoprotein(10ng)Lane C: Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP1 (mucin domain deleted) Protein (aa:Met1-Asp320)(10ng)Lane D: Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP-RBD Protein(10ng) SecondaryHRP Conjugated Goat-anti-Rabbit IgG (H+L) at 1/1000 dilutionPerformed under reducing conditions.Applications for EBOV GP Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EBOV GP
The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses. It is processed into GP1 and GP2 fragments which remain disulfide linked. GP1 is entirely extracellular while GP2 is a transmembrane protein. GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2. GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR. Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane.
Long Name
Ebola Virus Glycoprotein
Alternate Names
bovGP
Gene Symbol
GP
Additional EBOV GP Products
Product Documents for EBOV GP Antibody - Azide and BSA Free
Product Specific Notices for EBOV GP Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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