EBF-3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16254
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (99%), Rat (99%), Xenopus (95%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human EBF-3. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (95%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit EBF-3 Antibody - BSA Free (NBP2-16254) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for EBF-3 Antibody - BSA Free
![Western Blot: EBF-3 Antibody [NBP2-16254]](https://resources.bio-techne.com/images/products/EBF3-Antibody-Western-Blot-NBP2-16254-img0001.jpg "Western Blot: EBF-3 Antibody [NBP2-16254]")
Western Blot: EBF-3 Antibody [NBP2-16254]
Western Blot: EBF3 Antibody [NBP2-16254] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: EBF-3 Antibody [NBP2-16254]](https://resources.bio-techne.com/images/products/EBF-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16254-img0003.jpg "Immunocytochemistry/ Immunofluorescence: EBF-3 Antibody [NBP2-16254]")
Immunocytochemistry/ Immunofluorescence: EBF-3 Antibody [NBP2-16254]
Immunocytochemistry/Immunofluorescence: EBF-3 Antibody [NBP2-16254] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um![Immunoprecipitation: EBF-3 Antibody [NBP2-16254]](https://resources.bio-techne.com/images/products/EBF-3-Antibody-Immunoprecipitation-NBP2-16254-img0006.jpg "Immunoprecipitation: EBF-3 Antibody [NBP2-16254]")
Immunoprecipitation: EBF-3 Antibody [NBP2-16254]
Immunoprecipitation: EBF-3 Antibody [NBP2-16254] - Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 ug of EBF3 antibody [C1C3] Western blot analysis was performed using EBF3 antibody [C1C3] Easy Blot anti-Rabbit IgG was used as a secondary reagent.Applications for EBF-3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EBF-3
Long Name
Early B-cell Factor 3
Alternate Names
COE3, EBF3, OE-2
Gene Symbol
EBF3
UniProt
Additional EBF-3 Products
Product Documents for EBF-3 Antibody - BSA Free
Product Specific Notices for EBF-3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.