E-Cadherin Antibody (CL1170) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34475
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0010.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0014.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL1170
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P12830, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Rat (80%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for E-Cadherin Antibody (CL1170) - BSA Free
![Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CL1170-Western-Blot-NBP2-34475-img0016.jpg "Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475]")
Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475]
Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CL1170-Immunocytochemistry-Immunofluorescence-NBP2-34475-img0015.jpg "Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475]")
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475]
Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of BEWO cells using the Anti-CDH1 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0011.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0012.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0013.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]")
Applications for E-Cadherin Antibody (CL1170) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
UniProt
Additional E-Cadherin Products
Product Documents for E-Cadherin Antibody (CL1170) - BSA Free
Product Specific Notices for E-Cadherin Antibody (CL1170) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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