dsRNA Antibody Pack

Multi-Species

Affinity Purification, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Monoclonal

Mouse monoclonal antibodies NBP3-11395, NBP3-11396, and NBP3-11397 recognise double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by all three antibodies, although in some assays the affinity of NBP3-11395 and NBP3-11396 to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens. NBP3-11397 shows higher affinity to poly(I).poly(C) than to the other dsRNA antigens, although the difference of apparent binding constants may vary under different experimental conditions.

Antibodies in this pack are validated for the following applications:

NBP3-11397: ELISA, Flow Cytometry, Immunoaffinity Purification, Immunoblotting, Immunocytochemistry/Immunofluorescence, Immunohistochemistry
NBP3-11395: ELISA, Immunoaffinity Purification, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
NBP3-11396: ELISA, Immunoblotting, Immunocytochemistry/Immunofluorescence, Immunohistochemistry
NL007: Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.

As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). We therefore highly recommend centrifuging (microcentrifuge) the reconstituted antibody before use and using the supernatant..

Antibodies in this pack are validated for use in the following species:

NBP3-11397: Multi-Species
NBP3-11395: Multi-Species
NBP3-11396: Multi-Species
NL007: Mouse

Protein A purified

After reconstitution the mAbs will be in PBS without any stabilisers or preservatives at a concentration of 1 mg/ml

No Preservative

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

The lyophilised samples should each be reconstituted with 100 ul sterile distilled water.

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.