DOT1L Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-40845
![Western Blot: DOT1L Antibody [NB100-40845]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-Western-Blot-NB100-40845-img0007.jpg "Western Blot: DOT1L Antibody [NB100-40845]")
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Knockdown Validated
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis, Chip Cytometry, IF/IHC, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23861670).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit DOT1L Antibody (NB100-40845) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-DOT1L Antibody: Cited in 13 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for DOT1L Antibody
![Western Blot: DOT1L Antibody [NB100-40845]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-Western-Blot-NB100-40845-img0014.jpg "Western Blot: DOT1L Antibody [NB100-40845]")
Western Blot: DOT1L Antibody [NB100-40845]
DOT1L-Antibody-Western-Blot-NB100-40845-img0014.jpg![Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-Immunohistochemistry-Paraffin-NB100-40845-img0009.jpg "Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845]")
Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845]
Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation: DOT1L Antibody [NB100-40845] - Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of DOT1L binding within a 250 Kb region of chromosome 10 as detected using anti-DOT1L antibody.
![Western Blot: DOT1L Antibody [NB100-40845]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-Western-Blot-NB100-40845-img0015.jpg "Western Blot: DOT1L Antibody [NB100-40845]")
Applications for DOT1L Antibody
Application
Recommended Usage
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Chromatin Immunoprecipitation was reported in scientific literature (PMID:19734945). For ChIP-Seq: 4 ug/30 ug chromatin. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DOT1L
Long Name
Disruptor of Telomeric Silencing 1-like
Alternate Names
H3-K79-HMTase, KMT4
Entrez Gene IDs
84444 (Human)
Gene Symbol
DOT1L
UniProt
Additional DOT1L Products
Product Documents for DOT1L Antibody
Product Specific Notices for DOT1L Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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