DNAJB11 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84899

Novus Biologicals, part of Bio-Techne
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NBP1-84899
NBP1-84899-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNAJB11 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit DNAJB11 Antibody - BSA Free (NBP1-84899) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-DNAJB11 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DNAJB11 Antibody - BSA Free

Western Blot: DNAJB11 Antibody [NBP1-84899]

Western Blot: DNAJB11 Antibody [NBP1-84899]

Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: DNAJB11 Antibody [NBP1-84899]

Immunocytochemistry/ Immunofluorescence: DNAJB11 Antibody [NBP1-84899]

Immunocytochemistry/Immunofluorescence: DNAJB11 Antibody [NBP1-84899] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84899]

Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84899]

Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84899] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
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Applications for DNAJB11 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAJB11

Members of the heat shock protein 40 (HSP 40) family of proteins all contain a highly conserved J domain that associates with HSP 70 and regulates the function of HSP 70 by activating its adenosine triphosphatase activity. ERdj3, an HSP 40 chaperone, is expressed in the ER lumen, where it interacts with BiP, a molecule involved in retrotranslocating proteins out of the ER. ERdj3 also associates with several other protein substrates, including unfolded light chains, a nonsecreted Ig light chain mutant and a VSV-G ts045 mutant. Shiga toxin (Stx) is a bacterial tool that enzymatically inactivates the 28S rRNA, inhibiting protein synthesis of infected cells. Stx also interacts with ERdj3 and Sec 61 to form a complex through which proteins are retrotranslocated to the cytoplasm. ERdj3 may play a role in the ER quality control system.

Alternate Names

ABBP-2, APOBEC1-binding protein 2, DnaJ (Hsp40) homolog, subfamily B, member 11, dnaJ homolog subfamily B member 11, DnaJ protein homolog 9, EDJABBP2, ER-associated DNAJ, ER-associated dnaJ protein 3, ER-associated Hsp40 co-chaperone, ERdj3DJ9, ERJ3, ERj3p, HDJ9, hDj-9, HEDJERj3, Human DnaJ protein 9, PRO1080, PWP1-interacting protein 4, UNQ537

Gene Symbol

DNAJB11

Additional DNAJB11 Products

Product Documents for DNAJB11 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNAJB11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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