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DNA Polymerase epsilon catalytic subunit A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13067

Novus Biologicals, part of Bio-Techne
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NBP3-13067

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DNA Polymerase epsilon catalytic subunit A. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

262 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for DNA Polymerase epsilon catalytic subunit A Antibody

Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067]

Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067]

Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
DNA Polymerase epsilon catalytic subunit A Antibody

Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody (NBP3-13067) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
DNA Polymerase epsilon catalytic subunit A Antibody

Immunocytochemistry/ Immunofluorescence: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] -

POLE antibody detects POLE protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: POLE protein stained by POLE antibody (NBP3-13067) diluted at 1:2500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Applications for DNA Polymerase epsilon catalytic subunit A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:2500

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Polymerase epsilon catalytic subunit A

Participates in DNA repair and in chromosomal DNA replication

Alternate Names

DKFZp434F222, DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit A, EC 2.7.7, EC 2.7.7.7, POLE1FLJ21434, polymerase (DNA directed), epsilon

Gene Symbol

POLE

Additional DNA Polymerase epsilon catalytic subunit A Products

Product Documents for DNA Polymerase epsilon catalytic subunit A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Polymerase epsilon catalytic subunit A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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