DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-115

Novus Biologicals, part of Bio-Techne
Discontinued Product
NB100-115 has been discontinued. View all DNA Polymerase epsilon catalytic subunit A products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Drosophila (Negative), Mustelid, Primate

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3C5.1

Format

BSA Free

Concentration

1.0 mg/ml

View all DNA Polymerase epsilon catalytic subunit A Antibodies »

Product Specifications

Immunogen

Human DNA Polymerase epsilon purified from HeLa cells.

Reactivity Notes

Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.

Localization

Nuclear

Specificity

This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1)BSA Free [NB100-115]

Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1)BSA Free [NB100-115]

Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.

Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115]

Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of DNA Polymerase epsilon catalytic subunit A [3C5.1] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Applications

Application
Recommended Usage

Immunohistochemistry

reported in scientific literature (PMID 29935943)

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500

Western Blot

5 ug/ml
Application Notes
In WB, this DNA Polymerase epsilon (3C5.1) antibody detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DNA Polymerase epsilon catalytic subunit A

DNA Polymerase epsilon, also called DNA polymerase epsilon subunit 3 (POLE3) is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. DNA Polymerase epsilon generates a complex with DNA polymerase epsilon subunit CHRAC1 and associates with naked DNA, which is then incorporated into chromatin, facilitated through the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. DNA Polymerase epsilon is a component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4, wherein, the interaction with POLE4 is a prerequisite for further binding with POLE or POLE2. DNA Polymerase epsilon interacts with CHRAC1, and together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRA. DNA Polymerase epsilon histone-fold protein dimers combine within larger enzymatic complexes for facilitating the processes of DNA packaging, replication, and transcription.

Alternate Names

DKFZp434F222, DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit A, EC 2.7.7, EC 2.7.7.7, POLE1FLJ21434, polymerase (DNA directed), epsilon

Gene Symbol

POLE

Additional DNA Polymerase epsilon catalytic subunit A Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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