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DMAP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55093

Novus Biologicals, part of Bio-Techne
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NBP2-55093
NBP2-55093-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: AEEEYLLQELRKIEARKKEREKRSQDLQKLITAADTTAEQRRTERKAPKKKLPQKKEAEKPAVPETAGIKFPDFKSAGVTLRSQRMKLPSSVGQKKI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit DMAP1 Antibody - BSA Free (NBP2-55093) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DMAP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DMAP1 Antibody [NBP2-55093]

Immunocytochemistry/ Immunofluorescence: DMAP1 Antibody [NBP2-55093]

Immunocytochemistry/Immunofluorescence: DMAP1 Antibody [NBP2-55093] - Staining of human cell line HEK 293 shows localization to nucleoplasm & cytosol.
DMAP1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: DMAP1 Antibody - BSA Free [NBP2-55093]

Chromatin Immunoprecipitation-exo-Seq: DMAP1 Antibody - BSA Free [NBP2-55093]

ChIP-Exo-Seq composite graph for Anti-DMAP1 (NBP2-55093) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for DMAP1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DMAP1

DMAP1 (DNA methyltransferase 1 associated protein 1) has been identified as a subunit of several distinct protein complexes involved in the repression and activation of transcription. It has been observed to interact with histone deacetylase 2 (HDAC2) as well as histone acetyltransferase (HAT) indicating a role in nucleosome modification to regulate transcription. DMAP1 can act independently to repress transcription in association with DNA methyltransferase 1. Alternate names for DMAP1 include DNMT1-associated protein 1, DNMAP1, DMAP1, KIAA1425, EAF2, SWC4, DNMAP1, DNMTAP1, FLJ11543, and DKFZp686L09142.

Alternate Names

DNA methyltransferase 1 associated protein 1, DNA methyltransferase 1-associated protein 1, DNMAP1DNMT1-associated protein 1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425DKFZp686L09142, MEAF2, SWC4

Gene Symbol

DMAP1

Additional DMAP1 Products

Product Documents for DMAP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DMAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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