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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 185709

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Dkk-4
Leu19-Leu224
Accession # Q9UBT3

Specificity

Detects human Dkk-4 in ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant mouse (rm) Dkk-4 is observed and no cross-reactivity with recombinant human (rh) Dkk-1 or rhDkk-3 is observed. In sandwich immunoassays, less than 2% cross-reactivity with rmDkk-4 is observed and no cross-reactivity with rhDkk-1, recombinant rat Dkk-1, rmDkk‑1, or rhDkk-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Dkk-4 Antibody (185709) [Dylight 350]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Dkk-4

DKK4 is a secreted protein that is a member of the dickkopf family. Like some other members of the dickkopf family DKK4 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The activity of DKK4 is modulated by binding to the Wnt co-receptor and the co-factor kremen 2, and it is proposed that it is also an inhibitor of the Wnt signaling pathway.

Long Name

Dickkopf-4

Alternate Names

Dkk4

Gene Symbol

DKK4

Additional Dkk-4 Products

Product Documents for Dkk-4 Antibody (185709) [Dylight 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dkk-4 Antibody (185709) [Dylight 350]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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