Dityrosine Antibody (10A6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59361

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10A6

Format

BSA Free

Concentration

1 mg/ml

View all Dityrosine Antibodies »

Product Specifications

Immunogen

Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Dityrosine Antibody (10A6) - BSA Free (NBP2-59361) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Dityrosine Antibody (10A6) - BSA Free

Western Blot: Dityrosine Antibody (10A6) [NBP2-59361]

Western Blot: Dityrosine Antibody (10A6) [NBP2-59361]

Western Blot: Dityrosine Antibody (10A6) [NBP2-59361] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (NBP2-59361). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (NBP2-59361) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Immunocytochemistry/ Immunofluorescence: Dityrosine Antibody (10A6) [NBP2-59361]

Immunocytochemistry/ Immunofluorescence: Dityrosine Antibody (10A6) [NBP2-59361]

Immunocytochemistry/Immunofluorescence: Dityrosine Antibody (10A6) [NBP2-59361] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibodyat 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Flow Cytometry: Dityrosine Antibody (10A6) [NBP2-59361]

Flow Cytometry: Dityrosine Antibody (10A6) [NBP2-59361]

Flow Cytometry: Dityrosine Antibody (10A6) [NBP2-59361] - Flow Cytometry analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (NBP2-59361). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (NBP2-59361) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.
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Applications for Dityrosine Antibody (10A6) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Dityrosine

Alternate Names

Dityrosine;Dityrosine (DT);DT;DY

Additional Dityrosine Products

Product Documents for Dityrosine Antibody (10A6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dityrosine Antibody (10A6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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