Digoxigenin was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Digoxigenin labeled anti-Vimentin antibodies bound in the bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Digoxigenin in HeLa Human Cell Line.

Digoxigenin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm incubated with Digoxigenin-labeled anti-Vimentin antibody. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Digoxigenin Antibody

Application

Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and Digoxigenin labeled anti-Vimentin antibody

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and Digoxigenin labeled anti-Vimentin antibody

Reviewed Applications

Read 1 review rated 5 using MAB10386 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References

Décarie, A.A. et al. (1994) Peptides 15(3):511.
Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Alternate Names

DIG

Additional Digoxigenin Products

Product Documents for Digoxigenin Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Digoxigenin Antibody

For research use only

Citations
Reviews

Digoxigenin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10386

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Digoxigenin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Digoxigenin Antibody

Digoxigenin in Human Liver.