DENND5B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31971

Novus Biologicals, part of Bio-Techne
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NBP2-31971
NBP2-31971-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Mouse, Avian

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DENND5B Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FIWDFIEKVVAYFETTDQILDNEDDVLIQKSSCKTFCHYVNAINTAPRNIGK

Reactivity Notes

Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit DENND5B Antibody - BSA Free (NBP2-31971) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-DENND5B Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DENND5B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DENND5B Antibody [NBP2-31971]

Immunocytochemistry/ Immunofluorescence: DENND5B Antibody [NBP2-31971]

Immunocytochemistry/Immunofluorescence: DENND5B Antibody [NBP2-31971] - Staining of human cell line U-2 OS shows localization to nucleoli & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971]

Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971]

Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971]

Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971]

Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971] - Staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferus ducts.
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Applications for DENND5B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Knockout Validated

Knockout Validated from a verified customer review.

Western Blot

Validated for Western Blot from a verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-31971 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DENND5B

MGC24039( AAH20855, 1 a.a. - 508 a.a.) full-length recombinant protein with GST.

Alternate Names

DENN domain-containing protein 5B, DENN/MADD domain containing 5B, DKFZp686P1174, FLJ41648, FLJ43333, MGC24039, Rab6IP1-like protein

Gene Symbol

DENND5B

UniProt

Q6ZUT9 (Human)

Additional DENND5B Products

Product Documents for DENND5B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DENND5B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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