D-Dimer Antibody (28) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05045
![ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]](https://resources.bio-techne.com/images/products/D-Dimer-Antibody-28-BSA-Free-ELISA-NBP1-05045-img0004.jpg "ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Canine
Cited:
Human
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot, ELISA
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 28
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified human D-dimer
Epitope
The epitope appears to relate to the neo-epitope formed by cleavage of D-dimer from fibrin.
Specificity
Binds human D-dimer. Cross-reacts with directly coated D-monomer but not with directly
coated fibrinogen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for D-Dimer Antibody (28) - BSA Free
ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]
ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045] - The calibration curve of a sandwich assay for D-Dimer using NBP1-05045 as capture antibody and NBP2-23592 as the biotinylated detection antibody.Applications for D-Dimer Antibody (28) - BSA Free
Application
Recommended Usage
ELISA
1:8000
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-05045 binds free D-dimer in solution. NBP1-05045 works as a capture antibody in sandwich ELISA with biotinylated NBP1-05045 or NBP2-21616 as a detection antibody. Biotinylated NBP1-05045 works as a detection antibody in sandwich ELISA with NBP2-21616 or NBP1-05045 as a capture antibody. The combination with NBP1-05045 as a capture antibody and NBP2-23592 as a detection antibody is recommended.
WB: Due to reducing conditions in Western blotting NBP1-05045 binds the beta-chain (40kDa) of D-Dimer, and not the entire D-Dimer complex. Use in IHC-P reported in scientific literature (PMID:33480806)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: D-dimer
Long Name
D-Dimer breakdown product of Fibrinogen
Alternate Names
Ddimer, FDP
Additional D-dimer Products
Product Documents for D-Dimer Antibody (28) - BSA Free
Product Specific Notices for D-Dimer Antibody (28) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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