Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88039

Novus Biologicals, part of Bio-Techne
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NBP1-88039-25ul
NBP1-88039
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytochrome b5 Outer Mitochondrial Membrane Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVETSVTYYRLEEVAKRNSLKELWLVIHGRVYDVTRFLNEHPGGEEVLLEQAGVDASESFEDVGHSSDAREMLKQYYIGDIHPSDLKPESGSKD

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22203676), Mouse reactivity reported in scientific literature (PMID: 25144769).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free (NBP1-88039) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Cytochrome b5 Outer Mitochondrial Membrane Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP1-88039]

Immunocytochemistry/ Immunofluorescence: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP1-88039]

Immunocytochemistry/Immunofluorescence: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP1-88039] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP1-88039]

Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP1-88039]

Analysis in human cell lines A-549 and HeLa using Anti-CYB5B antibody. Corresponding CYB5B RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free Immunohistochemistry-Paraffin: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP1-88039]

Immunohistochemistry-Paraffin: Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free [NBP1-88039]

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
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Applications for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25144769). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome b5 Outer Mitochondrial Membrane

Alternate Names

CYB5M, CYB5-M, CYPB5M, Cytochrome b5 outer mitochondrial membrane isoform, cytochrome b5 type B, cytochrome b5 type B (outer mitochondrial membrane), DKFZp686M0619, OMB5, outer mitochondrial membrane cytochrome b5, type 2 cyt-b5

Gene Symbol

CYB5B

Additional Cytochrome b5 Outer Mitochondrial Membrane Products

Product Documents for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome b5 Outer Mitochondrial Membrane Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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